伯明翰大学PhD Position in Creating and characterising novel substrate-specific synthetic methyl-lysine sensors申请条件要求-申请方
主办方
伯明翰大学
PhD直招介绍
About the Project
Applications are invited for a 3-year PhD Studentship at the University of Birmingham starting in February 2027. This supramolecular chemical biology studentship is based in the laboratory of Dr Martin Higgs ( http://www.birmingham.ac.uk/research/cancer-genomics/research/lysine-methylation-dna-damage/index.aspx ) and will be co-supervised by Professor Andy Wilson in the School of Chemistry ( https://www.birmingham.ac.uk/staff/profiles/chemistry/wilson-andy ).
Lysine methylation is a critical post-translational modification occurring on both histones (epigenetic modifications) and non-histone proteins. A major roadblock in detecting lysine methylation is the reliance on antibodies or nanobodies against the methylated protein in question. This limits our understanding of when lysine methylation occurs on different proteins, and how these methylation events are controlled.
The aim of this PhD is to create new substrate-specific tools to detect lysine methylation and use them to investigate the dynamics of non-histone methylation. Specifically, the student will:
1: Adapt existing synthetic methyl-lysine receptors (e.g. sclx4) to create a tailored molecular methyl sensor: The candidate will first synthesise a series of sclx4 derivatives by addition of peptides predicted to bind the sequence/molecular surface surrounding methylated residues of a previously identified methylated DNA repair protein.
2: Develop a methyl-peptide sensing platform using competitive turn-on fluorescence : using the sensors synthesised above, the candidate will then optimise this fluorescence-based platform, which will be used to assess binding to methyl-peptides and methyl-proteins.
3: Investigate the dynamics of MRE11 methylation: The student will use this fluorescence reporter system to track changes in methyl-state of the DNA repair protein of interest upon induction of methylation and/or before/after DNA damage in cells. Finally, the student will use fluorescently labelled sensors in single molecule cellular assays to track methylation in real time.
This studentship will provide new tools to study lysine methylation with spatio-temporal resolution, yielding biological insight into how methylation is regulated.
References:
Hof. Host-guest chemistry that directly targets lysine methylation: synthetic host molecules as alternatives to bio-reagents. Chem Commun (Camb) 52, 10093-10108 (2016).
Bayley et al. H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ. Mol Cell 82, 1924-1939 e1910 (2022).
Yi et al. Discovery of SHANK1-PDZ Peptide-Fragment Inhibitors Using a Dynamic Ligation Screening Strategy, Biochemistry 65, 1202–1213 (2026),
伯明翰大学 PhD Position in Creating and characterising novel substrate-specific synthetic methyl-lysine sensors项目有没有奖学金，是不是全奖Phd招生，下面我们一起看一下【大学名称】Phd的奖学金资助情况
项目资助情况
Funding notes:
This is a three-year studentship with a standard UKRI stipend rate and tuition fees for UK students.
The project is open to both home and international students, although additional fees (~£3600 for visa and healthcare surcharge and ~£30,000/year for international tuition fees) will be required for non-UK applicants.
Please contact Dr Martin Higgs, and/ or Prof Andy Wilson for an informal discussion before applying.
伯明翰大学Phd申请条件和要求都有哪些？PhD Position in Creating and characterising novel substrate-specific synthetic methyl-lysine sensors项目是不是全奖？有没有奖学金？下面我们一起看一下伯明翰大学申请Phd直招需要具备哪些条件和要求，以及托福、雅思语言成绩要到多少才能申请。
申请要求
Person Specification:
Applicants should have a strong background in synthetic chemistry or chemical biology, and a clear interest in its application to the study of biomolecular mechanisms. They should be able to demonstrate prior competence in a laboratory environment. They should have a strong commitment to research in chemical biology, epigenetics and/or genome stability, and hold or realistically expect to obtain at least an Upper Second Class Honours Degree in Chemistry Biochemistry or suitably aligned topic.
报名方式
招生人信息
Dr M Higgs, Prof A Wilson
Professor Email:
m.r.higgs@bham.ac.uk
a.j.wilson.1@bham.ac.uk
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